Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
نویسندگان
چکیده
منابع مشابه
Ionic Permeability and Electrical Potential Differences in Necturus Kidney Cells
The cellular concentrations of Na, K, and Cl have been measured in kidney slices of the amphibian, Necturus maculosus. Permeability coefficients have been determined for Na, K, Cl, Rb, Cs, and choline, from studies both of the uptake of radioactive isotopes and the rate of cell swelling in anisotonic solutions. The results of both methods were found to agree well. Measurements were also made of...
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Stable electrical potential differences can be measured by means of conventional glass microelectrodes across the cell membrane of renal tubule cells and across the epithelial wall of single tubules in the doubly perfused kidney of Necturus. These measurements have been carried out with amphibian Ringer's solution, and with solutions of altered ionic composition. The proximal tubule cell has be...
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Many aspects of the glomerular filtration of macromolecules remain controversial, including the location of the major filtration barrier, the effects of electrical charge, and the reason the filtration barrier does not clog. We examined these issues in anesthetized Necturus maculosus, using fluorescently labeled probes and a two-photon microscope. With the high resolution of this system and the...
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This paper describes experiments designed to evaluate Na(+) and Cl(-) transport in isolated proximal straight tubules from rabbit kidneys. When the perfusing solution was Krebs-Ringer buffer with 25 mM HCO(3) (-) (KRB) and the bath contained KRB plus 6% albumin, net volume reabsorption (J(v), nl min(-1) mm(-1) was -0.46 +/- 0.03 (SEM); V(e), the spontaneous transepithelial potential difference,...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1961
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.44.4.689